Master projects available in the Stocker lab
Technology development in aquatic microbial ecology
Aquatic microbes inhabit an ever-changing environment, where they frequently battle predators, viruses, and each other during their quest for nutrients. A key aspect of this micro scale life is the ability to sense and navigate their surroundings, and many aquatic microbes tackle this challenge through swimming informed by chemical sensing. Traditionally, researchers have struggled to observe microbial behavior in the environment, due to a number of technical hurdles. Recently the Stocker Lab has developed several tools that allow measurements of these microbial processes in unprecedented detail at the micro scale; however, our current deployment methods limit the range of habitats we can access with these new technologies. The objective of this research project is to develop and construct a deployment technology for new micro devices designed to study micro scale microbial processes in aquatic environments. Such an advance will make possible the overall goal of understanding how aquatic microorganisms access resources and thereby shape the natural and man-made systems in which they live.
The Masters project will require design and hands-on work with a number of fabrication tools, including (but not limited to) 3D printing, laser cutting, and CNC milling in order to construct new deployment platforms, based on the specific deployment environment. In addition, the student has the possibility to carry out proof of concept experiments, for example to isolate bacteria that are dominant in the selected ecosystem. These isolates could later be used in an extensive bio prospecting campaign in an effort to discover new microbial functions that may be applicable to engineered systems (i.e. drinking water quality, wastewater treatment, bio remediation), with the potential to scale-up and impact human health and well-being. Device deployment could occur in one of several systems, including lakes, rivers, soil, wastewater treatment plants, and drinking water systems.
The candidate should be familiar with prototyping techniques and have some computer-aided design experience. Although previous exposure to environmental microbiology is not a requirement, enthusiasm to develop in this area of knowledge is desirable.
During their project, the student will have the opportunity to carry out some (or all) of the following work:
- Design and fabrication of a deployment platform for a chosen ecosystem;
- Plan experiments to isolate key microbes involved in selected environmental processes;
- Carry out an isolation campaign, whereby microorganisms are isolated from the environment and screened through a combination of molecular methods and microscopy.
For more information, contact Bennett Lambert (firstname.lastname@example.org).
Bacterial dispersion in groundwater
Understanding the transport of bacteria in saturated porous media is crucial for many applications in water management, ranging from the control of bio-clogging in pumping wells to the design of new bioremediation schemes for subsurface contamination. However, little is known about the spatial distribution of bacteria at the pore scale, particularly when small-scale heterogeneities – always present even in seemingly homogeneous aquifers – lead to preferential pathways for groundwater flow. In particular, the coupling of flow and motility has recently been shown to strongly affect bacterial transport, and this leads us to predict that subsurface flow may influence the dispersal of bacteria and the formation of biofilms in saturated aquifers. The specific scope of this project is to study bacterial dispersion in a sinusoidal microchannel, a simplified model that can provide great insight into the basic mechanisms that occur at the pore scale. Although natural media are in general not so simple (and certainly not periodic), the sinusoidal channel represents the simplest system to analyze the effect of the convergence and divergence of streamlines on dispersion, a mechanism that plays a central role for transport in heterogeneous porous media. Furthermore, at fracture scale, the channel model is relevant to understand the role of fracture wall roughness on transport properties.
In this project, the student will have the opportunity to learn the basics of numerical modeling and to become acquainted with microfluidic technology and optical microscopy applied to the study of bacterial transport. Flow in sinusoidal channels with different dimensions will first be studied with numerical methods (e.g., in COMSOL Multiphysics). Depending on the time available, the same geometries will then be modeled using microfluidic channels, where the transport of passive and active tracers will be visualized with an optical microscope and quantified with particle tracking techniques.
For more information, contact Eleonora Secchi (email@example.com) or Roberto Rusconi (firstname.lastname@example.org).
Investigation of diel changes in buoyancy and self-assembly in the important oceanic cyanobacteria Trichodesmium
In this project, the student will have the chance to generate fundamental insights into the mechanics and ecology of the ocean’s most prominent nitrogen-fixing microorganisms, the filamentous multicellular cyanobacteria of the genus Trichodesmium, which are responsible for one quarter of global nitrogen fixation. Behavior of Trichodesmium in its natural habitat is complex: single filaments self-assemble into two major types of millimeter-scale aggregate structures that likely confer ecological advantages under certain conditions, like iron and phosphate shortage. Aggregates and single filaments can vertically migrate in the water column by changing buoyancy, but only aggregates can get as deep as 150 m, because vertical speed is dependent on aggregate size. Such behavior may allow them to scavenge for phosphate at depth during the night and photosynthesize at the surface during daylight, and may thus play a fundamental role in their contribution to nitrogen fixation.
The aim of this project is to develop an automated 24h-imaging setup that can be used to quantify diel (day-night cycle) changes in aggregation behavior (for example change in aggregate volume with time) and buoyancy (sinking/rising speed), to carry out imaging under several experimental conditions, and, depending on the time available and interests of the student, to analyze quantitatively the resulting videos.
During the project, the student will gain experience in fabrication, including laser cutting, to create a small enclosure for controlled imaging studies, handling of harmless bacterial cultures, and video imaging and analysis. After fabrication of an experimental imaging chamber, the student will carry out experiments to determine the influence of iron and phosphate concentration on buoyancy and self-assembly dynamics under different light regimes and at different stages of growth. The student should be able to work mainly independently on fabrication of the imaging chamber and optimization of the imaging itself, which will be carried out with remotely controlled digital SLR time-lapse photography, possibly involving two cameras. Quantitative video analysis will require some Matlab skills. An interest in the ecological framework of this study is desirable.
An example video showing aggregation of positively buoyant Trichodesmium in a non-optimal set up can be seen here.
For more information, contact Ulrike Pfreundt (email@example.com).
Groundwater on a chip: study of double porosity/permeability using microfluidics
A porous medium consists of interconnected pores (voids or cavities) in a solid material through which a fluid (liquid/gas) can flow. Often, porous media show a bimodal distribution of pore sizes, having macropore and micropore regions. Examples occur in nature in a wide range of biological (tissues, blood vessels) and inorganic (soil, sedimentary rocks) materials, with a long-standing interest for areas such as hydrology (soils and aquifers), petroleum engineering (oil/gas reservoirs), chemical engineering (filters and reactors), and bio-mechanics.
There is evidence that flow and transport processes in this type of porous medium often cannot be described using classical models, which assume uniform flow and transport, and a large number of more complex models have been proposed. The aim of this project is to develop a microfluidic device as a model of double porous media, and study fluid flow and transport experimentally and through numerical analysis.
During the project, the student will have the opportunity to carry out some of the following:
- Design and microfabrication to develop a microfluidic device to represent a porous medium with bimodal pore size distribution;
- Perform experiments to visualize and quantify anomalous (non-equilibrium) flow and transport through the micromodel;
- Develop a numerical model and perform simulations to study the flow field and transport through the micromodel.
For more information, contact Mehdi Salek (firstname.lastname@example.org) or Joaquin Jimenez-Martinez (email@example.com).
Chemical flow tracing: micro-optode facilitated O2 sensing in aquatic ecosystems
Fluid motion is commonly measured via particle image velocimetry (PIV), in which passive tracer particles added to the fluid (or naturally present within it) allow the visualization and quantification of flow field. While this methodology provides rich data on the physical aspects of fluid motion (e.g. streamlines, flow velocities), it does not provide information on the chemical environment. Chemical measurements, on the other hand, are usually limited to point measurements (using electrochemical or optochemical sensors) or 2D arrays (via planar optode materials), which typically fail to capture the complex and dynamic processes that are the direct result of the physical microenvironment. Thus neither approach in its present implementation can track fluid flow and the chemical microenvironment concurrently, yet such information is vital to understand microscale interactions that are dictated by the interplay between biotic and abiotic factors.
The project will tackle this problem by developing the method of ‘chemically-enabled particle image velocimetry’ (cPIV) to obtain simultaneous information on chemical microenvironments and flow fields. The student will gain hands-on experience in using O2-sensing (“micro-optode”) tracer particles in microfluidic devices, in microscopy, and in image analysis. Together, these technologies will allow the student to perform a stepwise calibration of O2-sensing particles in prescribed gas-gradients and fluid flows. This calibration procedure will involve a careful analysis of the resulting image data to infer physical and chemical processes simultaneously. Once the system is calibrated, the student will use cPIV to detect O2 heterogeneities on complex biological surface environments (for example, coral fragments) and permit a first real-time estimation of mass-transfer processes in aquatic environments.
For more information, contact Lars Behrendt (firstname.lastname@example.org).
Development of a microfluidic sorting device for the isolation of polysaccharide-degrading bacteria
Polysaccharides are the main building blocks of plant, insect and bacterial biomass. In fact, most of the bio-carbon on our planet is stored in the form of polysaccharides such as cellulose, lignin, chitin and peptidoglycan. Bacteria are one of the main degraders of polysaccharides in nature and thus are key players in the global carbon cycle. Once a bacterial population encounters a specific polysaccharide, whether in the soil, in the ocean, or even in the intestine of another organism, the bacterial population will produce enzymes to degrade and consume it. Polysaccharides have a complex structure, which requires a complex set of reactions in order to be used, and often requires several bacteria species to complete the degradation. However, up until now we do not have a simple method to identify and isolate the bacteria responsible for the degradation of a specific polysaccharide among the many species in a specific environment. In this project, we aim to develop a novel microfluidic device to identify and isolate the bacteria that use a specific polysaccharide within a complex environment. Besides the potential applications of the device in environmental/green industry, this project is part of a large effort to understand the role of bacteria in the carbon cycle of oceans and lakes.
The student will gain experience in a variety of methods, including microfabrication, organic synthesis, microscopy and experimental microbiology. During the project, they will have the opportunity to carry out some (not necessarily all) of the following:
- development of a novel microfluidic sorting device;
- use of electron microscopy to characterize microbe behavior in the device;
- proof of concept experiments using mixtures of known bacteria;
- DNA sequencing and bacterial diversity analysis.
The candidate should be highly motivated and independent. For more information, contact Uria Alcolombri (email@example.com).
Development of a robotic approach to study bacteria in turbulence
The aim of this project is to establish a robotic approach to directly probe the behavioral dynamics of marine microbes under the influence of turbulent stirring. The student will have the opportunity to develop and construct a device with robotic arms to stir a nutrient resource, program the robotic arm to stir the fluid at different rates and with different motion patterns, and use an existing image acquisition system to visualize the resulting bacterial population dynamics in real time. Students do not need prior knowledge in robotics, but a passion for hands-on work and fabrication is a plus. The ultimate goal is to determine how turbulence affects bacterial growth rate, which is a vital component of marine element cycles and thus one of the keystones of global climate.
For more information, contact Yutaka Yawata (firstname.lastname@example.org).